WS21:Methods in Life Sciences
Abschnittsübersicht
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Start of Lecture Series
The first lecture of the series will take place on Mo, 18.10.2021, 8:15.
See below for information and material regarding the individual lectures.
"Methods" lectures are always on Mondays and Tuesdays.Delivery of lectures
- Lectures will take place in person at the lecture hall, 00.006, Beatrice-Edgell-Weg 21
- In addition, we will have an interactive, live transmission of every lecture via "Zoom" for those who cannot attend in person (see below). By "cannot" we mean that you are still abroad because you did not get a visa in time or that you are in quarantine or similarly good reasons. We do not mean that you forgot to get up in time ;-)
Onyl attending in person will give you the opportunity to network with the lecturers and peers after each lecture.
Regulations at the lecture hall
- Only
students who are either fully vaccinated against SARS-CoV2, have
recovered from the virus or are tested to be virus negative will be
admitted.
- You
must wear a mask when you enter the building, and we recommend (or may
even require under some circumstances) that you keep it on even after
sitting down at the lecture hall.
- Please make sure to arrive at the lecture hall well before 8:15 (at least 10 min), because we will not admit anyone after 8:15 and you have to check in at the lecture hall via the UniNow App.
Online rules
- Join with your real and full name, and have/keep your webcam switched on. If you do not comply with that rule we will remove you from the Zoom meeting. Should there be technical reasons why you cannot join with a live image, you have to give a reason for that via the chat function before the start of the lecture
- Make yourself familiar with the Zoom platform and preferentially use the Chrome browser
- Access "Zoom" exclusively via: https://uni-wuerzburg.zoom.us; when there, klick on "Teilnahme - Meeting beitreten"
The following meeting link will remain valid for the entire semester (or until further notice): Meeting-ID: 934 5522 9952; Password: 643803 - It may happen that some lecturers will speak online. We will however always transmit the lecture to the lecture hall, so even if the professor does not come in person,
you can watch the lecture at the lecture hall.
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We are happy to receive any feedback about the "Methods" lecture series. Please use the link or enter the questionaire using the QR-Code below.
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Mo 25.4.2022, 17.30-18.30
GSLS Lecture Hall, 00.006, Beatrice-Edgell-Weg 21
Registration on WueStudy from 16.3. to 15.4.2022
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I would like to know, how many of you are still inclined to come to the lecture hall to attend the lectures in person. Please participate in the poll.
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Print it, set a timer, work on it for 60 min if you do both "Topics" and "Methods", and only 30 min if you just do the "Methods" questions.
We meet (if you like) online at 10 am, 22.12. via the ususal lecture link to briefly discuss.
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25.10.2021; 08:15-10:00 am
Lecturer: Christian Stigloher
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02.11.2021; 08:15-10:00 am
Lecturer: Philip Kollmansberger
Contents: Basics of image formation, filtering and denoising, segmentation, machine learning, particle tracking, colocalization, relevant tools
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08.11.2021; 08:15-10:00 am
Lecturer: Lars Schönemann, Head of Recombinant Protein Expression Facility @ RVZ
Contents: This lecture will give a comprehensive overview about todays most commonly used methods and techniques when it comes to the production and purification of recombinant proteins.
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09.11.2021; 08:15-10:00 am
Lecturer: Hermann Schindelin
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15.11.2021; 08:15-10:00 am
Lecturer: Andreas Schlosser
Contents: Mass Spectrometry Basics, Electrospray Ionization (ESI), Principles of Mass Analyzers (Orbitrap), Database searching, Quantitative Proteomics (e.g. SILAC), Posttranslational Modifications (PTMs), ID of protein interaction partners
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16.11.2021; 08:15-10:00 amLecturer: Hans MaricContents: Protein-protein interactions (PPIs) form the molecular basis for most physiological and pathophysiological processes in cells and the human body. This lecture will introduce state-of-the-art methods and technologies for quantifying PPIs and discuss strategies for therapeutic targeting of PPIs.Outline:
- thermodynamics and kinetics of PPIs
- specific methods for quantifying PPIs
- limitations and perspectives for targeting PPIs
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23.11.2021; 08:15-10:00 am
Lecturer: Matthias Wolf
Note: The lecturer will appear via Zoom; we will transmit the lecture to the lecture hall
Contents:Please refer to the following link for recapitulating the lecture
https://www.jove.com/de/t/3806/the-its2-database
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29.11.2021; 08:15-10:00 am
Lecturer: Lars BarquistContents: High-throughput sequencing, genome assembly and analysis, bacterial applications of RNA-seq, CLIP-seq, transposon insertion sequencing -
30.11.2021; 08:15-10:00 amLecturer: Lars DölkenContents: Overview on sequencing technologies; Metabolic labelling of newly transcribed RNA; Ribosome Profiling; Single cell sequencing (scRNA-seq) and its combination with metabolic labeling (scSLAM-seq)The lecture had to be cancelled, but Professor Dölken has sent his slides. Although they are not relevant for the exam, we recommend you have a look, as there will certainly be questions regarding sequencing methods on the exam, and those slides might help to get a better understanding of what you heard in principle also in some of the other lectures.
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- development (embryonic cell proliferation, larval imaginal discs)
- genetics (genome, gene names, balancers)
- methods:
- transgenesis (P-element mediated, PhiC31-Integrase-mediated)
- CRISPR (background & use, mutagenesis, misexpression; gene drive)
- binary overexpression (GAL4/UAS, QF/QUAS, overexpression & RNAi)
- mosaicism (FLP/FRT, mitotic clones, flp-out clones, examples)
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- principles of genetic effectors to manipulate neuronal activity- principles of genetic strategies to trace neurons and neuronal circuit connectivity- principles of genetically encoded indicators of neuronal activity- showcase the genetic power of Drosophila as a model in neuroscience- builds on the lecture "Drosophila Toolbox I"
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21.12.2021; 08:15-10:00 amLecturers: Manik ChatterjeeNote: The lecturer will appear via Zoom; we will transmit the lecture to the lecture hallContents:
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11.01.2022, 08:15-10:00 am
Lecturer: Tomasz Jüngst
Postponed to 7.2. -
17.01.2022, 08:15-10:00 am
Lecturer: Carsten Scheller
Note: The lecturer will appear via Zoom; we will transmit the lecture to the lecture hall -
18.01.2022; 08:15-10:00 amLecturer: Chase BeiselNote: The lecturer will appear via Zoom; we will transmit the lecture to the lecture hall
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24.01.2022; 08:15-10:00 am
Lecturers: Flavio Roces and Keram Pfeiffer
Note: The lecturers will appear via Zoom; we will transmit the lectures to the lecture hall
Contents: Measuring behavior; Respirometry; Electrophysiology
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25.01.2022; 08:15-10:00 am
Lecturer: Wolfgang Rössler
Note: The lecturer will appear via Zoom; we will transmit his lecture to the lecture hall
Contents:
- insect brains and behavior
- quantitative neuroanatomy
- molecular imaging and neurotracing
- multi-unit electrophysiology
- in-situ calcium imaging
- learning paradigms
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07.02.2022; 08:15-10:00 am
Lecturer: Tomasz Jüngst
Postponed from original date, 11.01.2022
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08.02.2022; 08:15-10:00 am
Lecturer: Juliano Sarmento Cabral
Note: The lecturer will appear via Zoom; we will transmit his lecture to the lecture hall